Descripción
Rat Interleukin 6 (IL-6) ELISA Kit
FOR RESEARCH USE ONLY. Not for clinical diagnosis use
Catalog #:31238
Introduction
l For the quantitative determination of rat IL-6 concentrations.
l Detection of species: Rat
l Detection medium: serum, plasma, tissue homogenates, cell culture supernates.
Principle of test
The kit is for the quantitative level of IL-6 in the sample, adopt purified rat IL-6 to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to IL-6, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody – antigen – enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of IL-6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
RANGO DE DETECCIÓN
Rango de detección del kit es: 8 ng / L -150ng / L
Valoraciones
No hay valoraciones aún.