Rat Vascular Endothelial Growth Factor,VEGF ELISA Kit

Descripción

Rat Vascular Endothelial Growth Factor,VEGF ELISA Kit

The kit is for the quantitative level of VEGF in the sample, adopt purified rat VEGF to
coat microtiter plate, make solid-phase antibody, then add samples or standards to
wells with a labeled antibody specific to VEGF, then add labeled HRP to the well. After
washing completely, add TMB substrate solution, TMB substrate becomes blue
color in wells that contains antibody – antigen – enzyme-antibody complex, reaction
is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of VEGF in the samples is then determined
by comparing the O.D. of the samples to the standard curve.