Rat Tumor necrosis factor ?,TNF-? ELISA KIT

Descripción

Rat Tumor necrosis factor ?,TNF-? ELISA KIT

The kit is for the quantitative level of TNF-α in the sample, adopt purified rat TNF-α to
coat microtiter plate, make solid-phase antibody, then add samples or standards to
wells with a labeled antibody specific to TNF-α, then add labeled HRP to the well. After
washing completely, add TMB substrate solution, TMB substrate becomes blue
color in wells that contains antibody – antigen – enzyme-antibody complex, reaction
is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of TNF-α in the samples is then determined
by comparing the O.D. of the samples to the standard curve.