Rat adiponectin,ADP ELISA Kit

Descripción

Rat adiponectin,ADP ELISA Kit

The kit is for the quantitative level of ADP in the sample, adopt purified rat ADP to coat
microtiter plate, make solid-phase antibody, then add samples or standards to wells
with a labeled antibody specific to ADP, then add labeled HRP to the well. After
washing completely, add TMB substrate solution, TMB substrate becomes blue
color in wells that contains antibody – antigen – enzyme-antibody complex, reaction
is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of ADP in the samples is then determined by
comparing the O.D. of the samples to the standard curve.