ACHI elisa kit

Descripción

ACHI elisa kit

Principle of the assay
The kit assay ACHI level in the sample, use Purified ACHI antibody to coat microtiter
plate wells, make solid-phase antibody, then add ACHI to wells, Combined ACHI antibody
which With HRP labeled, become antibody – antigen – enzyme-antibody complex, after washing
Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP
enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the
color change is measured spectrophotometrically at a wavelength of 450 nm. The
concentration of ACHI in the samples is then determined by comparing the O.D. of the samples
to the standard curve.
Materials provided