Rat Transforming Growth factor ?1,TGF-?1 ELISA kit

Descripción

Rat Transforming Growth factor ?1,TGF-?1 ELISA kit

The kit is for the quantitative level of TGFb1 in the sample, adopt purified rat TGFb1 to
coat microtiter plate, make solid-phase antibody, then add samples or standards to
wells with a labeled antibody specific to TGFb1, then add labeled HRP to the well. After
washing completely, add TMB substrate solution, TMB substrate becomes blue
color in wells that contains antibody – antigen – enzyme-antibody complex, reaction
is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of TGFb1 in the samples is then determined
by comparing the O.D. of the samples to the standard curve.