Rat Testoterone,T ELISA Kit

Descripción

Rat Testoterone,T ELISA Kit

The kit is for the quantitative level of Testoterone in the sample, adopt purified Rat
Testoterone to coat microtiter plate, make solid-phase antibody, then add samples or
standards to wells with a labeled antibody specific to Testoterone, then add labeled
HRP to the well. After washing completely, add TMB substrate solution, TMB
substrate becomes blue color in wells that contains antibody – antigen –
enzyme-antibody complex, reaction is terminated by the addition of a stop solution
and the color change is measured at a wavelength of 450 nm. The concentration of
Testoterone in the samples is then determined by comparing the O.D. of the samples to
the standard curve.