Descripción
rat follicle-stimulating hormone,FSH ELISA Kit
The kit is for the quantitative level of FSH in the sample, adopt purified Rat FSH to coat
microtiter plate, make solid-phase antibody, then add samples or standards to wells
with a labeled antibody specific to FSH, then add labeled HRP to the well. After
washing completely, add TMB substrate solution, TMB substrate becomes blue
color in wells that contains antibody – antigen – enzyme-antibody complex, reaction
is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of FSH in the samples is then determined by
comparing the O.D. of the samples to the standard curve.
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