Rat ovalbumin specific IgE,OVA sIgE ELISA Kit

Descripción

Rat ovalbumin specific IgE,OVA sIgE ELISA Kit

The kit is for the quantitative level of OVA sIgE in the sample, adopt purified rat OVA sIgE to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to OVA sIgE, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody – antigen – enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of OVA sIgE in the samples is then determined by comparing the O.D. of the samples to the standard curve.